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Dried hydrophilic serum extracts were reconstituted in potassium phosphate buffer in D 2 O (690 L, 100 mM, pH 7.4) and TSP (10 L, sodium 3-trimethylsilyl-propionate-2,2,3,3,-d4) as chemical shift reference ( 0.0) (98 atom % D, Sigma-Aldrich, Milan)

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